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Figure 2


Fig. 2. Live imaging of a cultured neuron reveals spine lengthening after activin treatment. (A,B) Fluorescence images of dendrites of a cultured hippocampal neuron. pEGFP-transfected neurons were treated with activin (100 ng/ml; B) or vehicle (A) and observed for 6 hours. Upper and lower panels are before and after treatment, respectively. Arrowheads indicate spines whose shape changed by 6 hours. Scale bar, 10 µm. (C,D) Length change of individual spines before and after treatment; 38 control spines were treated with BSA (C), and 40 spines were treated with activin (D).