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Figure 4


Fig. 4. Immunostaining of EBs with ExE markers. Anti-COUP-TF1 (A), anti-Gata4 (B), anti-Krt19 (C) and anti-Foxa2 (D) antibodies were used for immunostaining of EBs grown with (+) or without (–) Tet in LIF-supplemented medium. Two independent clones gave similar results. Bars, 100 µm.