Fig. 5. The temporal expression pattern of ExE markers during EB differentiation. After culture in LIF-supplemented medium with or without Tet for several passages, ESCs with the inducible Sox17 transgene were allowed to aggregate and were grown in the same medium for the indicated periods. Additionally, the parental ESCs were allowed to aggregate and were grown with or without LIF for the indicated periods. The expression levels relative to those in EBs derived from parental ESCs grown for 6 days are shown as the mean values of duplicate experiments. The Sox17 primer set was designed to detect both endogenous and exogenous Sox17.