Fig. 4. Effect of Dia1 knockdown on the distribution of - and -catenin. Immunofluorescence of cells transfected with a GFP-encoding vector together with either an empty pSuper vector (A,B and E,F) or pSuper si-hDia1 (C,D and G,H) stained with antibodies against -catenin (B,D) and -catenin (F,H). Arrows indicate cell-cell junctions. Note that both - and -catenins are enriched and uniformly distributed along the cell-cell junctions in control cells (B,F), whereas junctions between Dia1-knockdown cells show reduced and fragmented staining (D,H). Bar, 10 µm.