Fig. 5. Effect of Dia1 knockdown on the localization of VASP and phosphotyrosine. Immunofluorescence of cells transfected with a GFP-encoding vector together with either an empty pSuper vector (A-C,G-I) or pSuper si-hDia1 (D-F,J-L) stained with antibodies against -catenin (B,E,H,K), phospho-tyrosine (C,F) and VASP (I,L). Note that Dia1 deficiency leads to disappearance of these components from the cell-cell interface. Bars, 10 µm. Arrows indicate the contacts between cells.