Fig. 7. Activation of exogenous Dia1 leads to increased recruitment of E-cadherin to cell-cell junctions. (A,B) Confocal images of cells co-transfected with both GFP-mDia1- and RhoA-V14-expressing vectors and stained for E-cadherin 20 hours following transfection. A prominent junctional localization of GFP-mDia1 (A) and E-cadherin (B) is evident. (C,D) XZ projections of the images (A) and (B), respectively, taken along the white lines. Junctions between transfected cells are indicated by arrows. Bar, 10 µm. (E) Assessment of the width of the `adhesion belt' in control cells and in cells co-transfected with vectors encoding GFP-mDia1 and RhoA-V14. The lengths of cell-cell contacts at the XZ plane are indicated. Quantification was done for 30 pairs of cells for each experiment. Error bars represent s.d. values. The asterisk indicates the value differs significantly from corresponding controls (Student's t-test, P<0.0001).