Fig. 8. Localization of Dia1 and truncated mutant Dia1 proteins. (A) Structures of full-length mDia1 and its constitutively active mutants that were used in this study. The black lines indicate the region encoded by each mutant. The numbers correspond to the amino acid positions in the mDia1 sequence. None of the mutants contains an intact C-terminus. Only the CFP-DAD mutant preserves the N-terminal domain in its entirety. (B) Diffuse distribution of the Dia1 mutant GFP-N3 and staining of actin (C) and -catenin (D) in the corresponding cells. (E) Junctional localization of the Dia1 mutant CFP-DAD. (F) Co-transfection of GFP-N3 together with a vector encoding RhoA-V14 promotes the localization of the Dia1 mutant GFP-N3 to cell-cell junctions. (G) Cells expressing the mutant CFP-DAD co-transfected together with RhoA-V14 display a prominent mDia1-DAD localization to cell-cell junctions (as well as to tips of filopodia). Arrows indicate cell-cell junctions. Bar, 10 µm.