Fig. 5. Effect of TLK1 and AUK1 double knockdown on spindle formation and chromosome segregation in T. brucei. The effect of TLK1 and AUK1 double knockdown was compared with those from TLK1 or AUK1 single knockdowns. (A) Northern blot with AUK1, the 5'-UTR of TLK1 or -tubulin as probes. (B) Comparison of growth of TLK1 and AUK1 double knockdown cells with those of the single knockdown cells. (C) Effect of AUK1 and TLK1 double knockdown on spindle formation. The 1N2K and 2N2K cells were labeled with the KMX-1 antibody (left panel) and examined for spindle structures. The data are presented as the mean percentage±s.e. of 200 cells counted from three independent experiments (right panel). (D) Double knockdowns of TLK1 and AUK1 enriched the 1N2K cells by 10-20% in comparison with those from the single knockdowns. The data are presented as the mean percentage±s.e. of 200 cells from three separate experiments. Bar, 2 µm.