Fig. 6. The two Asf1 homologs are required for promoting S-phase progression in T. brucei. (A) Single and double knockdowns of Asf1A and Asf1B in T. brucei. A northern blot was performed with Asf1A, Asf1B or
-tubulin sequences as probes. (B) Effects of the single and double knockdowns on cell growth. (C) Effects of the single and double knockdowns on cell cycle progression. The percentages of cells at G1-, S- and G2/M-phases were determined and plotted against time post induction.