(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. GBF1 depletion causes COPI dissociation from membranes. (A) Human GBF1 isoforms showing insertions ({triangledown}) and deletions ({Delta}). Shown are the positions of the two siRNAs and the two antibodies against GBF1 used in this study. Both siRNAs target all GBF1 isoforms. Both antibodies recognize epitopes present in all GBF1 isoforms. HeLa cells (B-D,F) or NRK cells (E) were untreated (`con'; control), transfected with reagent alone (`mock'), transfected with siRNA against GBF1 (`siRNA') or transfected with scrambled siRNA (`scr'). After the indicated times (B,C) or after 72 hrs (D,E), cell lysates were western blotted with antibodies against the indicated proteins. GBF1 is efficiently and selectively depleted from HeLa and NRK cells. (F) HeLa cells were transfected with siRNA against GBF1 for 72 hours and then stained with the indicated antibodies. Depletion of GBF1 correlates with dissociation of β-COP. Bars, 16 µm.