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Figure 2


Fig. 2. GBF1 depletion causes tubulation of the cis-Golgi. (A-G) HeLa cells transfected with #2siRNA against GBF1 for 72 hours were stained with antibodies against the indicated proteins. Depletion of GBF1 does not cause collapse of the ERGIC and the Golgi but leads to extensive tubulation of the cis-Golgi. The level of cis-Golgi tubulation correlates with the level of GBF1 depletion (G). (H) HeLa cells transfected with #1siRNA against GBF1 for 72 hours were stained with antibody against GM130. Analogous extensive tubulation of the cis-Golgi is observed with siRNAs #1 and #2. Bars, 16 µm.