Fig. 7. Comparison of INPP5B and OCRL1 at 15°C. HeLa cells transiently expressing GFP-INPP5B or GFP-OCRL1 (green) were incubated at 15°C for 3 hours before fixation in methanol and labelling with antibodies against ERGIC53 or the transferrin receptor (TfR) (red). Bar, 10 µm. Insets show a magnified view of the boxed area.