Fig. 1. (A) Immunofluorescence for topo II on HTETOP metaphase cells grown in the absence (topo II^ON) and presence (topo II^OFF) of dox for 48 hours. (B) Immunofluorescence for the SMC2 subunit of condensin on HTETOP metaphase spreads isolated from cells grown in the presence and absence of dox for 72 hours. The normal axial distribution of SMC2 is detected following depletion of topo II. Bar, 10 µm.