Fig. 6. The effect of depletion of topo II on anaphase PICH-coated threads. HTETOP cells expressing topo II (A), or depleted of topo II (48 hours exposure to dox) (B) were fixed and permeabilised before being co-stained for PICH (green) and CENP-B (red). Shown are cells in anaphase or undergoing aberrant cytokinesis. Arrowheads indicate examples of PICH threads that are not associated with DAPI-stained chromatin bridges. Bar, 10 µm. (C) Quantification of the number of PICH threads for anaphase cells expressing topo II (topo II^ON) and for cells treated for 48 hours with dox (topo II^OFF). 50 anaphase cells were analysed for each, and the stage was determined by the distance between segregating sister kinetochores.