Fig. 3. The effect of MK-TRAP on the proliferation of G401-derived cell lines in monolayer culture. (A) G401-derived cell lines M1 and M2 that stably overexpress MK-TRAP, and control cell lines C1 and C2 were seeded into 96-well dishes and then counted with a cell counting kit over 4 consecutive days. The growth rate was calculated as the ratio of the measured OD on each day to the OD on day one. n=8; error bars, ± s.e.m.; ****P<0.001 (MK-TRAP cells versus control cells). (B) Exogenous MK (100 ng/ml) was added to assess its effect on the growth of M1 cells versus control (C1) cells. n=8; error bars, ± s.e.m.; ***P<0.005; ****P<0.001.