Fig. 6. NCAM140 is recycled in growth cones and neurites of cortical neurons. Cortical neurons isolated from E15.5 mice (NCAM^+/–) were transiently transfected with NCAM140. Endocytosis was induced for 1 hour by application of an NCAM-specific antibody. Cells were fixed and processed for immunofluorescence analysis. NCAM was visualized using secondary Cy3-conjugated antibodies, Rab11 was detected by Rab11-specific antibodies and Alexa-Fluor-488-conjugated secondary antibodies. Images were taken from growth cones or neurites of cortical neurons using a confocal laser scanning microscope.