Fig. 5. Immunocytochemical analysis of C2C12 cells for myogenin and MHC expression. C2C12 cells were maintained for 72 hours under differentiation conditions in the presence or absence of SDF1 (10 ng/ml) and subsequently stained with antibodies against myogenin and MHC (MF20) as indicated. Trypan Blue was used for counterstaining. Note that SDF1 clearly reduces the number of myotubes that express myogenin or MHC. Bar, 100 µm.