Fig. 3. Myo52 function and movement are not dependent upon its neck region. (a) Myo52 protein with boxed IQ-motif sequences. Residues deleted from GFP-Myo52IQ are underlined. (b) Yeast two-hybrid assay showing interaction of the Myo52 tail with itself or the fission yeast calmodulin Cam1. (c) Anti-GFP western blot of myo52-strain extracts expressing Myo52-nGFP, GFP-Myo52IQ or GFP-Myo52CC confirm the expected reduced size of the Myo52 mutant in the latter strain. (d,e) Micrographs of (d) Myo52CC and (e) Myo52IQ cells show that only Myo52IQ localised normally and was able to complement the morphology defect associated with the myo52 allele. (f) Kymographs of Myo52IQ cells demonstrate that the truncation mutant can make rapid directed and bidirectional movements throughout the cell. (g) Velocity distributions of Myo52 and Myo52IQ. Bars, 10 µm.