Fig. 6. WAVE2 regulates polarisation of the Golgi apparatus. (A) Histogram showing the number of NIH 3T3 cells with polarised Golgi apparatus following treatment with siRNA targeting either: nothing (cont), WAVE1 (W1), WAVE2 (W2) or both. Data from three separate experiments were collated. *** indicates a P value <0.0001 (Fisher's exact test) for the null hypothesis that there is no difference between WAVE- and control-knockdown cells. Percentage figures indicate the percentage of cells with a polarised Golgi apparatus. P, polarised Golgi; NP, nonpolarised Golgi. (B) Typical immunoblot analysis of protein levels following treatment with siRNA. (C) Visualisation of the Golgi apparatus (antibody against GM130) in green, F-actin (phalloidin) in red and nuclei (DAPI) in blue following control siRNA (cont) or WAVE2 siRNA. The wound edge is oriented vertically at the right of each panel. The lower panel shows GM-130 staining alone. Bar, 20 µm.