Fig. 5. A combination of Rip11 siRNA duplexes blocks insulin-stimulated uptake of 2-deoxyglucose but not transferrin binding by 3T3-L1 adipocytes. 3T3-L1 adipocytes were electroporated with control siRNA duplex or a combination of Rip11A and Rip11B siRNA duplexes (Rip11A/B) at two different concentrations directed to deplete Rip11. The cells were left for 48 hours before measuring: (A): uptake of 2-deoxyglucose in the absence or presence of 100 nM insulin. (B) The level of expression of Rip11 mRNA, which was measured using quantitative real-time PCR with the data shown relative to the level of expression of mRNA encoding cyclophilin A, which was itself unchanged by the Rip11 siRNA. (C) ¹²⁵I-labelled transferrin binding in the absence or presence of 100 nM insulin. Each panel is representative of three separate preparations of adipocytes, with the data expressed as the mean ± s.e.m.