Fig. 1. TGF
1 modulation of FAK phosphorylation. (A) Whole lysates of TGF
1-treated cells were analyzed for FAK expression (top panel) and specific phosphorylation on tyrosine residues 397 or 925 (bottom panels) by immunoblotting. (B) Mesangial cells serum-deprived for 24 hours were treated with TGF
1 (right panels) or vehicle (left panels) for 30 minutes, then fixed and stained for phospho-Y397 FAK (pY397, top) or phospho-Y925 FAK (pY925, bottom). Bar, 20 µm.