Fig. 6. Effects of FAK mutants on Elk-Gal-reporter and–0.42(I) collagen promoter activity induced by TGF1. (A) Mesangial cells were transfected with the Elk-Gal reporter system as described for Fig. 4C, along with an expression vector for either wild-type FAK (WT) or point mutants (Y397F or Y925F) of FAK, and TGF1 induction of ERK activity was evaluated as Elk-mediated luciferase activity. (B)–0.42(I) collagen promoter activity was stimulated by TGF1 in the presence of either a wild-type or point-mutant (Y397F or Y925F) FAK. A representative result (mean ± s.e.m.) performed in triplicate from one of four separate experiments is shown. Values were corrected for -galactosidase expression. Fold induction by TGF1 in each condition is shown in the graphs. White bars, vehicle; black bars, TGF1. *P<0.05, compared to each control by a post-hoc analysis.