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Figure 6


Fig. 6. Photoactivated localization microscopy (PALM) imaging of focal adhesions near the coverslip surface in paraformaldehyde-fixed Gray fox lung fibroblast cells. (A) Widefield fluorescence image of multiple focal adhesions labeled with tdEos fused to human vinculin. (B) Total internal reflection fluorescence (TIRF) summed-molecule image of the focal adhesion within the region indicated by the box in A. (C) PALM view of the focal adhesion structure shown in B, which includes the apparent assembly of vinculin into a partial network (arrows).