Fig. 4. Interaction of Tyr and Tyrp1 in cycloheximide-treated melanocytes. (A) Confocal images of melan-a melanocytes treated with or without cycloheximide. Melanocytes expressing YFP-ER or YFP-Golgi were treated with cycloheximide (10 µg/ml) for 10 hours, fixed and stained with the Tyr_GP antibody as described for Fig. 1. Melanocytes treated with cycloheximide were also double-stained with Tyr_R, Tyrp1_GP or HMB45 as noted. Bright-field images are shown to view pigment granules in the cells. Bar, 10 µm. (B) Interaction of Tyr and Tyrp1 in cycloheximide-treated melan-a melanocytes was analyzed by chemical crosslinking with DSP, immunoprecipitation and immunoblotting, as described for Fig. 2.