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Figure 5


Fig. 5. ALK4 co-localizes with VSMCs in yolk sacs from ALK5–/– embryos. (A-F) Cryosections of yolk sacs from wild-type embryos at E9.5 stained with antibodies against ALK4 (B,E; red) in combination either with PECAM as a marker of ECs (A; green) or SMA{alpha}, as a marker of VSMCs (D; green). The overlays (C,F) show that ALK4 is expressed in VSMCs (F) and, to a lesser extent, in ECs. (G) The number of SMA{alpha}-positive cells compared to the number of endoderm cells, as an internal control, in individual yolk sacs (*P<0.05). EC, endothelial cell layer; end, endoderm; mes, mesothelial cell layer.