Fig. 7. ALK4 signalling is active in ALK5–/– EBs. Smooth muscle actin was detected by immunohistochemistry in sectioned EBs, stained as whole mounts. No striking difference was observed in SMA
expression between wild-type (A,A') and ALK5–/– (B,B') EBs, and, after incubation with SB-431542, SMA
was no longer detected in both wild-type (C,C') and ALK5–/– EBs (D,D'). Scale bars, 0.5 mm.