Fig. 3. LMTK2 and myosin VI colocalise in cultured cells. (A) HeLa cells were co-transfected with untagged LMTK2 and GFP-tagged myosin VI, and labelled with a (a,a') polyclonal antibody against LMTK2 and (b,b') a monoclonal antibody against GFP. Panels a-c are confocal z-stacks, panels a'-c' are magnifications of a single confocal slice of the boxed regions in a-c. (B) Wide field images are shown of RPE cells that were transfected with LMTK2-GFP and labelled with (d,d') monoclonal antibody against GFP and (e,e') polyclonal antibody against myosin VI. Panels d'-f' are magnifications of the boxed regions in d-f. Bars, 10 µm.