Fig. 4. LMTK2 is present in the endocytic compartments. (a-c) HeLa cells transfected with LMTK2-GFP were loaded with Tf–Alexa-Fluor-555 for 15 minutes, fixed and processed for immunofluorescence with anti-GFP antibody. (d-j) HeLa cells transfected with untagged LMTK2 were immunolabelled for (d,g) LMTK2 and (e) Rab5 or (h) EEA1. (k-m) HeLa cells stably expressing GFP-Rab11 were transfected with untagged LMTK2 and labelled with antibodies to LMTK2 and GFP. Merged images show LMTK2 colocalisation with the marker proteins in yellow. Single confocal slices (a-c,k-m) and wide field images (d-j) are shown. Insets represent enlarged images of the boxed regions. Bar, 10 µm.