Fig. 5. Depletion of myosin VI and LMTK2 changes the morphology and distribution of transferrin-positive endosomes. (A-C) HeLa cells were transfected twice with siRNA targeting myosin VI or LMTK2, or with non-specific control siRNA. (A,B) Two days after the second transfection the cells were harvested and processed for (A) western blotting with antibodies against LMTK2, myosin VI and -tubulin. In parallel experiments, cells were immunolabelled for (B) EEA1 and Vps26 or (C) loaded with Tf–Alexa-Fluor-555 before fixation. Insets represent enlarged images of the boxed regions. All images are confocal z-projections. Bars, 10 µm.