Fig. 6. Depletion of myosin VI or LMTK2 inhibits delivery of TfR to the Rab11-positive perinuclear recycling compartment. (A) HeLa cells stably expressing GFP-Rab11 were transfected with siRNA targeting myosin VI or LMTK2 and processed for immunofluorescence with antibodies against (a,d,g) TfR and (b,e,h) GFP, and labelled with DAPI to visualise nuclei. Merged images are shown in c, f and i. Bar, 10 µm. (B) HeLa cells transfected with siRNA targeting myosin VI or LMTK2 were pulsed with Tf–Alexa-Fluor-647 at 37°C for 30 minutes, washed and incubated at 37°C in the presence of excess of unlabelled transferrin. The amount of Tf–Alexa-Fluor-647 per cell was determined by FACS analysis. Data are presented as the mean ± s.e. from three independent experiments, each performed in duplicate.