Fig. 7. Depletion of myosin VI and LMTK2 leads to reduction in Rab11-positive tubule formation. HeLa cells stably expressing GFP-Rab11 were transfected with siRNAs targeting myosin VI or LMTK2 and processed for immunofluorescence with anti-GFP antibodies. (A) A cell displaying a representative GFP-Rab11 distribution is shown for mock-transfected cells and for cells transfected with siRNA targeting myosin VI or LMTK2. Whereas multiple tubules emanate from the juxtanuclear region in mock-transfected cells (arrows), an almost complete lack of tubules was observed in both knockdowns. (B) Quantification of the number of tubules in mock-transfected or KD cells is shown. At least 500 cells were counted for the control group and for each RNAi experiment, and scored as either containing or not containing tubules. The results are expressed as percentage of cells containing tubules (mean ± range from two independent experiments, each performed in triplicate). Bar, 10 µm.