Fig. 5. Loss of MiDASes results in the failure of cytokinesis. Dividing HS1 cells expressing GFP-actin were observed by confocal microscopy. When one of the MiDASes (arrows) disappeared by accident, the other half of the cell resorbed this half, and eventually the cell failed to complete cytokinesis (A). The arrows indicate the position of the MiDAS in the resorbed half of the cell. (B) Time-course of area of left (filled squares) and right (filled triangles) halves, which are divided by a line in panel A. Note that the area of the resorbing half (open arrowhead) rapidly decreased after the disappearance of the MiDAS (closed arrowhead). Also, when the disappearance of MiDASes was artificially induced by treatment with thiabendazole, all of the cells (n=26 cells) failed to divide (C). Bars, 10 µm.