Fig. 3. Localization of Lsm7p upon depletion of other Lsm proteins. (A) Localization of Lsm7p in the absence of Lsm8p in the strain MPS7. MPS7 (P_GAL1-HA-LSM8) cells were grown in galactose medium to mid-log-phase and then shifted to glucose for 12 hours to deplete Lsm8p (confirmed by western blotting; data not shown). Nuclei depleted of Lsm7p are indicated by a large arrow and cytoplasmic foci are indicated by small arrows. (B) Localization of Lsm7p in strains lacking other Lsm proteins. lsm1 and lsm6 strains (MPS6 and MPS4) were grown at permissive temperature in glucose, whereas MPS8, MPS9 and MPS10 were depleted of Lsm3p, Lsm4p and Lsm5p, respectively, by growth in glucose for 12 hours. Representative cells are shown. Bars, 10 µm.