Fig. 5. Effect of Cep68 and Cep215 depletion on other cohesion proteins. U2OS cells were transfected for 48 hours with siRNA duplexes specific for Cep68 (A) or Cep215 (B) and then co-stained with the antibodies indicated (left columns) and antibodies against -tubulin (centre columns). Columns on the right show merged images with DAPI staining of DNA in blue, -tubulin in red and the various proteins in green. Bars, 10 µm.