Fig. 6. Mutual dependencies of cohesion-regulatory centrosomal proteins. U2OS cells were transfected for 48 hours with siRNA duplexes specific for rootletin (A), C-Nap1 (B) or pericentrin (C) and then co-stained with the antibodies indicated (left columns) and antibodies against -tubulin (centre columns). Columns on the right show merged images with DAPI staining of DNA in blue, -tubulin in red and the various proteins in green. Bars, 10 µm.