Fig. 2. Expression of SvkA during development and induction of cytofission. (A) Western blot with antibodies against SvkA showing the presence of the kinase throughout the 24 hours of wild-type development (2x10⁵ cells per lane). (B) The knockout cells grown on a plastic surface were considerably larger than the wild-type cells and the rescue cells. (C,D) SvkA-knockout cells were shifted from HL-5 medium to low-salt phosphate buffer (PB) and observed for several hours. The large and multinucleated cells separated only partially. Arrows indicate persistent connections between cells. Bars, 50 µm (B,C); 10 µm (D); see also supplementary material Movie 1.