Fig. 4. Paxillin is necessary for OL differentiation. (A) Primary OPCs were infected with paxillin103, paxillin1206 or luciferase shRNA retroviral vectors. At 3 days after induction of differentiation, lysates from infected cells were immunoblotted with antibodies against paxillin, Cdk5 and β-actin. (B) Representative fluorescence images of MBP and GFP-positive cells. Arrows indicate GFP-expressing cells. (C) Percentages of MBP-positive cells (red) against the total number of infected cells. Data were evaluated using Student's t-test (^*P<0.01).