Fig. 1. Uptake of mBSA-Texas-Red by clathrin-mediated endocytosis in Drosophila S2 cells. (A) Schematic diagram of the assay. (B) Representative images of cells treated with RNAi and assayed for uptake of mBSA-Texas-Red. Maximum projection of several Z-sections collected using a wide-field fluorescence microscope is shown. Dynamin, -adaptin and Chc images have approximately the same number of cells per field as control ones (not shown). (C) Fluorescence levels in cells treated with RNAi against known or proposed endocytic proteins. Quantification was performed using data from 3-4 experiments, each averaged from 10-12 images (average integrated fluorescence per cell), normalised using untreated cells (`Water') in the same experiment, and compared statistically with untreated cells. In this and all subsequent figures, ^*P<0.05, ^**P<0.01, ^***P<0.005, Student's t-test; all other comparisons are not significant (NS); error bars indicate s.e.m.