Fig. 5. Proliferation and signalling defects in vps35 mutant haemocytes. (A) Mutant vps35 third instar larvae develop melanotic masses. One WT and two vps35 animals are shown. (B) Representative DIC images of haemocytes isolated from WT or vps35 third instar larvae. Note increased numbers of small round cells (plasmatocytes) in vps35 larvae, and flat lamellocytes (arrows) that are absent in WT haemolymph. (C) Increased numbers of haemocytes extracted from vps35/Df larvae, relative to WT, and rescue using Hml-Gal4. Numbers are normalised relative to WT; n=12-15 images from six larvae; mean ± s.e.m. (D) Toll signalling is upregulated in vps35 mutant haemocytes, judged by increased levels of Toll (arrowhead indicates leading edge of lamellopodia that is labelled with Toll in vps35 but not in WT cell) and nuclear localisation of Dorsal. Dashed circles demarcate nuclei (as defined by bright field image). (E) Toll levels per cell; n=14 cells from five larvae. (F) Percentage of cells in which Dorsal is enriched in nuclei. n
300 cells isolated from six larvae; mean ± s.e.m.