Fig. 1. NCAM and FGFR1 colocalize at the cell surface. (A,B) NIH-3T3 cells were co-stained with antibodies against NCAM (red) and FGFR1 (green), followed by fluorochrome-conjugated secondary antibodies. After fixation, cells were subjected to TIRF analysis as described in the Materials and Methods. (B) Magnification of the boxed area in A. Arrows indicate the widespread, tight association of NCAM with FGFR1. Bars, 10 µm (A) and 0.2 µm (B).