Fig. 3. NCAM is sufficient for the regulation of the cellular response to FGF. (A) L cells stably transfected with empty vector (L-mock), full-length NCAM or FN2 were stimulated for 10 minutes with FGF2, followed by cell lysis and immunoblotting for phospho-ERK1/2 and then for total ERK1/2. (B) L cells stably transfected with empty vector, full-length NCAM or FN2 were left untreated or were treated with FGF2 for the indicated time periods. Cell proliferation was assessed as described in the Materials and Methods. The growth curves of untreated L-NCAM and L-FN2 cells, which were very similar to that of untreated L-mock cells (see also supplementary material Fig. S4A), have been omitted for simplicity. ^*P<0.005, relative to untreated L-mock cells.