Fig. 8. Localization of AtCLASP to mitotic arrays. (A) YFP:AtCLASP expressed from the native promoter in dividing root cells, MTs are labeled by expression of CFP:TUA4. On the right is the merged image with YFP:AtCLASP in the red channel and CFP:TUA4 in the green. Note prominent colocalization of YFP:AtCLASP and MTs at the PPB and relatively low signal intensity of YFP:AtCLASP on the spindle MTs. (B) Cells labeled as in A undergoing cytokinesis. Note that phragmoplast MTs are prominently labeled by CFP:TUA4 and almost undetectable by YFP:CLASP. Arrowheads indicate the preprophase band; asterisks depict an anaphase cell with spindle; arrows point to phragmoplast MTs. Scale bars: 10 µm.