Fig. 6. Effect of PLD isoform selective depletion on myogenic differentiation. The cells were transfected with isoform-specific siRNAs. The cells were switched from proliferation medium (10% FBS) to differentiation medium (1% FBS + 10^-7 M AVP), and myogenin immunofluorescence was assessed. The means ± s.e.m. of the percentages of myogenin-positive cells are shown in the graph (n=10 fields). Western blotting analyses were performed with PLD1- and PLD2-specific antibodies to assess the efficacy of siRNA silencing.