Fig. 4. SgIF4G-eIF4E association following fertilization, but not SgIF4G modification, is sensitive to rapamycin treatment. (A) SgIF4G-eIF4E association is inhibited by rapamycin. Affinity-purified protein (using an m⁷GTP column) obtained prior to or at the indicated times after fertilization from untreated (left) or 20 µM rapamycin-treated (right) eggs were subjected to western blot analyses using anti-SgIF4G (top), anti-human 4E-BP2 (bottom) and anti-eIF4E (middle) antibodies. (B) SgIF4G modification is not affected by rapamycin. The total amount of SgIF4G from untreated (left) or rapamycin-treated (20 µM) eggs (right), obtained prior to and at the indicated times after fertilization, was analysed by western blotting using anti-SgIF4G antibody (top). Anti-actin was used as loading control (bottom).