Fig. 2. Localisation of Spc110¹³p in wild-type cells. (A) Diagram summarising the key domain features of the SPB component Spc110p (Kilmartin et al., 1993; Geiser et al., 1993; Friedman et al., 1996; Stirling et al., 1996; Adams and Kilmartin, 1999; Elliot et al., 1999; Friedman et al., 2001) and depicting the predicted product of the high-dosage suppressor spc110¹³. CBD, calmodulin-binding domain; nls, nuclear localisation signal. (B) Localisation of full-length Spc110p-GFP or truncated Spc110¹³p-GFP in wild-type cells. Pairs of DIC and fluorescence images are shown. Spc110p-GFP localised to the SPBs throughout the cell cycle whereas Spc110¹³p-GFP localised uniformly to the nucleus. Bars, 2 µm.