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Figure 2


Fig. 2. Most cholesterol colocalizes with Golgi markers GM130 and giantin in BHK cells expressing wild-type or no CFTR, but not in cells expressing {Delta}F508 CFTR. GM130 was detected with mouse anti-GM130 mAb followed by goat anti-mouse Alexa Fluor 568 IgG conjugate; giantin was detected by rabbit anti-giantin antibody followed by goat anti-rabbit Alexa Fluor 568 IgG conjugate; and cholesterol was stained with filipin. In the overlay panels, GM130 and giantin are shown in red and filipin staining is shown in green.