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Figure 3


Fig. 3. Cell shape effects on the phosphorylation status of p190RhoGAP. (A) Immunoblots showing (a) tyrosine phosphorylated p190RhoGAP detected by phosphotyrosine antibody (4G10) or (b) p190RhoGAP immunoprecipitated with phosphotyrosine antibody (PY20) from total cell lysates of spread, cyto D-, or PP2-treated cells (*P<0.01). (B) Tyrosine phosphorylated p190RhoGAP detected by phosphotyrosine antibody (4G10) in TrX-100-soluble and -insoluble fraction isolated from spread versus cyto D-treated cells. (C) Raft localization of p190RhoGAP and Src tyrosine kinase in spread versus PP2-treated cells, using methods shown in Fig. 2C.