Fig. 2. TbCMF knockdown is specific and mutants can be placed into four phenotypic classes. (A) Classification of TbCMF knockdown mutants. The majority of TbCMF mutants exhibit cytokinesis defects that result in the formation of multicellular clusters (white arrows). Mutants were placed into one of four classes based on the size and time of formation of these clusters. A representative example of each mutant class is shown. Each panel shows a phase-contrast image of the indicated TbCMF knockdown strain grown in the absence (uninduced) or presence (induced) of 0.3 µg/ml tetracycline for five days. (B) Northern blots of RNA from two sample TbCMF mutants from each mutant class. RNA samples were probed with the gene targeted by RNAi and with a non-target gene as a loading control. ^*For TbCMF 2, equal loading was confirmed using rRNA visualized by UV.