Fig. 2. Rac1, via Basket, induces Rho1 activity. (A-E) The hemocyte actin cytoskeleton was visualized using Alexa Fluor 546-phalloidin (red) and the nucleus was stained with DAPI (blue). (A) Hml-Gal4 controls. (B) UAS-Rac1^F37A;Hml-Gal4;UAS-Rho1^N19. (C) Hml-Gal4;UAS-Rac1^N17.(D) Hml-Gal4;UAS-Rac1^N17/UAS-Rho1^V14; arrow indicates possible cytokinesis cleavage furrow. (E) Hml-Gal4;UAS-Rac1^N17/UAS-RhoGAPp190^rnai. (F) Determination of plasmatocyte diameter. The cell diameter of plasmatocytes from the various genotypes was measured, as described in Materials and Methods, and the diameter (µm) for 25 hemocytes was plotted. Different letters indicate similar groups (i.e. `a' is significantly different than `b' or `c' and so on. Student's t-test, P<0.01). (G) Percentage of multinucleate cells. The hemocytes from five different larvae were counted to determine the percentage of multinucleate cells as determined by DAPI staining [(number of multinucleate hemocytes/total number of hemocytes) x 100]. An asterisk indicates a significant difference (Student's t-test, P<0.01) compared with the parental UAS and Hml-Gal4 strains