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Figure 3


Fig. 3. Effect of tumor cell supernatant-derived BSG on MMP expression in PSC. (A,B) As demonstrated by zymography (A) and western blot (B), BSG isolated from MiaPaCa2, Panc1 and SW850 stimulates MMP-2 and MMP-1-expression in PSC to the same extent as complete supernatant. Final concentration of purified BSG and BSG in supernatant were adjusted to the same concentration. Supernatant depleted of BSG by immunoprecipitation (ID supernatant) did not increase MMP levels compared with control.